HSP27 antibody (pSer15)

Catalog No. ABIN3182033

The Rabbit Polyclonal anti-HSP27 antibody has been validated for WB, IHC and ELISA. It is suitable to detect HSP27 in samples from Human and Monkey.

Quick Overview for HSP27 antibody (pSer15) (ABIN3182033)

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSP27 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-HSP27 Antibody

Binding Specificity: pSer15

Specificity: Phospho-HSP27 (S15) Polyclonal Antibody detects endogenous levels of HSP27 protein only when phosphorylated at S15.

Characteristics: Rabbit Polyclonal to Phospho-HSP27 (S15).

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human HSP27 around the phosphorylation site of S15.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:100-1:300, ELISA 1:20000,

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Store at -20°C.

Target Details for HSP27

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Alternative Name: HSP27

Molecular Weight: 26 kDa

Gene ID: 3315

UniProt: P04792

Pathways: MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling