Nerve Growth Factor antibody

Catalog No. ABIN318395

The Rabbit Polyclonal anti-Nerve Growth Factor antibody is suitable to detect Nerve Growth Factor in samples from Human and Mouse. It has been validated for WB, Func and EIA.

Quick Overview for Nerve Growth Factor antibody (ABIN318395)

Target: Nerve Growth Factor (NGF)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nerve Growth Factor antibody is un-conjugated

Application: Western Blotting (WB), Functional Studies (Func), Enzyme Immunoassay (EIA)

Product Details anti-Nerve Growth Factor Antibody

Specificity: This antibody recognizes both the 2.5S and 7S forms of Mouse NGF beta, a secreted peptide involved in the development and maintenance of the sympathetic and sensory nervous systems. In a standard bioassay, this antibody neutralises the activity of 3.0 ng/mL NGF 2.5S when used at a concentration of around 0.5 μg/mL.

Purification: Affinitv Chromatography

Immunogen: Affinity purified Mouse 2.5S NGF

Isotype: IgG

Application Details

Application Notes: ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1.0 mL distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. We recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09 % Sodium Azide is recommended. For Functional Studies do not Add Sodium Azide.

Concentration: 1.0 mg/mL

Buffer: PBS, None, None

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store undiluted at -20 °C.

Target Details for Nerve Growth Factor

Target: Nerve Growth Factor (NGF)

Alternative Name: NGF

Background: Nerve growth factor (NGF) is one of a family of neurotrophins that induce the survival and proliferation of neurons. In cell culture, NGF induces the formation of neurite projections and, in vivo, may stimulate the innervation of tissues. NGF plays a role in the repair, regeneration, and protection of neurons, and as such could serve as a therapeutic agent in neurodegenerative conditions such as Alzheimer's disease. NGF enhances survival, growth, neurotransmitter biosynthesis of sympathetic and sensory neurons, neurotrophic factor, cutaneous innervation, growth, differentiation and survival of B lymphocytes. It also has a possible role in allergy and tissue repair. NGF is found in the hypothalamus, pituitary, thyroid gland, testes, epididymis, vascular smooth muscle cells, fibroblasts, mast cells and eosinophils. NGF is upregulated by glutamate, vitamin D3, IL6, FGF basic, astrocyte specific IL1, TNF alpha, PDGF and TGF beta. It is downregulated by GABAergic neuronal activity, glucocorticoids and Schwann cell-specific TGF beta.Synonyms: Beta-NGF, Beta-nerve growth factor, NGFB

Gene ID: 4803

NCBI Accession: NP_002497

UniProt: P01138

Pathways: Regulation of Cell Size