LHB antibody

Catalog No. ABIN318593

The Sheep Polyclonal anti-LHB antibody is suitable to detect LHB in samples from Human. It has been validated for ELISA, RIA and IRMA.

Quick Overview for LHB antibody (ABIN318593)

Target: LHB (Luteinizing Hormone beta Polypeptide (LHB))

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This LHB antibody is un-conjugated

Application: ELISA, Radioimmunoassay (RIA), Immunoradiometric Assay (IRMA)

Product Details anti-LHB Antibody

Specificity: This antibody recognises human luteinizing hormone, a gonadotrophic hormone found in the anterior lobe of the pituitary gland. TSH: 20 % hCG: 6 % FSH: 6 % Prolactin: 0.1 % hPL: 0.1 %

Purification: Purified IgG prepared by affinity chromatography on Protein G

Immunogen: Highly purified human pituitary luteinizing hormone.

Isotype: IgG

Application Details

Application Notes: IRMA. Radioimmunoassays: >1/50,000 initial dilution in 125I RIA overnight assay. ELISA: Has been used for coating and also as a capture antibody in a two site assay inconjunction with other monoclonal antibodies.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 5.0mg/mL

Buffer: Phosphate buffered saline, pH 7.2 containing 0.09 % Sodium Azide (NaN3)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for LHB

Target: LHB (Luteinizing Hormone beta Polypeptide (LHB))

Alternative Name: Luteinizing Hormone beta (LSH-Beta)

Target Type: Hormone

Background: Luteinizing Hormone is a member of the glycoprotein hormone family. Glycoprotein hormones are heterodimers consisting of a common alpha subunit and an unique beta subunit which confers biological specificity. LH is expressed in the pituitary gland and promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids. Mutations in the Luteinizing Hormone (LHB) gene are associated with hypogonadism which is characterized by infertility and pseudohermaphroditism.Synonyms: LH

Gene ID: 3972

NCBI Accession: NP_000885

UniProt: P01229

Pathways: Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity, C21-Steroid Hormone Metabolic Process