Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))
Specificity
This antibody is specific for porcine luteinizing hormone and does not react with GH, TSH, LH alpha or prolactin. Minimal reactivity has been observed with FSH.
LHB
ELISA, D, LF, IA, T
Host: Mouse
Monoclonal
unconjugated
Application Notes
Immunohistochemistry on frozen sections: 1/200 - 1/500. Western Blot: 1/300 - 1/400. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Reconstitution
Reconstitute with 50 μL sterile distilled water. For long term storage the addition of a preservative is recommended.
Handling Advice
Avoid repeated freezing and thawing.
Storage
-20 °C
Storage Comment
Prior to and following reconstitution store the antibody at -20 °C.
Luteinizing Hormone is a member of the glycoprotein hormone family. Glycoprotein hormones are heterodimers consisting of a common alpha subunit and an unique beta subunit which confers biological specificity. LH is expressed in the pituitary gland and promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids. Mutations in the Luteinizing Hormone (LHB) gene are associated with hypogonadism which is characterized by infertility and pseudohermaphroditism.