DLL4 antibody (Internal Region)

Catalog No. ABIN318838

This Rabbit Polyclonal antibody specifically detects DLL4 in WB, IHC (p) and EIA. It exhibits reactivity toward Human, Mouse, Rat and Monkey and has been mentioned in 4+ publications.

Quick Overview for DLL4 antibody (Internal Region) (ABIN318838)

Target: DLL4 (delta-Like 4 (DLL4))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DLL4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-DLL4 Antibody

Binding Specificity: Internal Region

Specificity: This antibody detects human Delta-like ligand 4 (DLL4), also known as Delta-4.

Purification: Purified

Immunogen: Synthetic peptide corresponding to the internal region of human DLL4.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry on paraffin sections: 5 - 15g/mL. ELISA: 1: 3000 - 1: 15000. Western Blot: 1: 300 - 1: 1500, detects a band of approximately 74 kDa in pancreatic celllysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0mg/mL

Buffer: Phosphate buffered saline pH 7.2 containing 0.09 % Sodium Azide (NaN3)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-DLL4 antibody (ABIN318838)

Wang, Lu, Zhang, Du, Tang, Wang, Lu, Hu: "GEN-27, a Newly Synthetic Isoflavonoid, Inhibits the Proliferation of Colon Cancer Cells in Inflammation Microenvironment by Suppressing NF-κB Pathway." in: Mediators of inflammation, Vol. 2016, pp. 2853040, (2017) (PubMed).

Li, Yan, Wang, Jing, Li, Liu, Yu, Fan: "Effects of canonical NF-κB signaling pathway on the proliferation and odonto/osteogenic differentiation of human stem cells from apical papilla." in: BioMed research international, Vol. 2014, pp. 319651, (2014) (PubMed).

Villaamil, Gallego, Valladares-Ayerbes, Caínzos, Aparicio: "Multiple biomarker tissue arrays: A computational approach to identifying protein-protein interactions in the EGFR/ERK signalling pathway." in: Journal of molecular signaling, Vol. 7, Issue 1, pp. 14, (2012) (PubMed).

González-Mariscal, Garay, Quirós: "Identification of claudins by western blot and immunofluorescence in different cell lines and tissues." in: Methods in molecular biology (Clifton, N.J.), Vol. 762, pp. 213-31, (2011) (PubMed).

Target Details for DLL4

Target: DLL4 (delta-Like 4 (DLL4))

Alternative Name: delta-Like Protein 4 / DLL4

Background: DLL4 is a homologue of the Drosophila delta protein, and functions as a transmembrane bound ligand to the Notch receptor Notch1. DLL4 is expressed in the vasculature and plays a critical role in vascular development. It is induced by vascular endothelial growth factor (VEGF), as a negative feedback regulator to regulate angiogenic sprouting and promote the formation of a differentiated vascular network. DLL4 has been found to be strongly expressed in tumour vessels of clear-cell renal tumours and bladder cancer, and inhibition of DLL4 results in increased vascular proliferation but defective maturation. This in turn leads to a decrease in tumour growth, with no apparent toxicity.Synonyms: Delta 4, Drosophila Delta homolog 4

Gene ID: 54567

NCBI Accession: NP_061947

UniProt: Q9NR61

Pathways: Notch Signaling