RAD9A antibody (AA 1125-1139)

Catalog No. ABIN318843

The Rabbit Polyclonal anti-RAD9A antibody (ABIN318843) specifically detects RAD9A in WB and EIA. The antibody is reactive with Saccharomyces cerevisiae samples.

Quick Overview for RAD9A antibody (AA 1125-1139) (ABIN318843)

Target: RAD9A (RAD9 Homolog A (S. Pombe) (RAD9A))

Reactivity: Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD9A antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-RAD9A Antibody

Binding Specificity: AA 1125-1139

Purification: Purified

Immunogen: Synthetic peptide corresponding to aa 1125-1139 of yeast Rad9 protein conjugated to Keyhole Limpet Haemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes: ELISA: 1: 2000 - 1: 4000. Western Blot: 1: 50 - 1: 250, detects a band of approximately 60 kDa.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5mg/mL

Buffer: Phosphate buffered saline pH 7.2 containing 0.09 % Sodium Azide (NaN3)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for RAD9A

Target: RAD9A (RAD9 Homolog A (S. Pombe) (RAD9A))

Alternative Name: RAD9A

Background: In S. cerevisiae this checkpoint is mainly controlled by the PI(3) kinase-like kinase (PIKK) Mec1, which is activated in response to DNA damage. Activated Mec1 hyperphosphorylates Rad9, which in turn triggers the binding of Rad53, the orthologue of mammalian Chk2. Activated Rad53 specifically targets substrates required for cell cycle arrest, gene expression and efficient DNA repair. Mutations affecting Rad9 impair checkpoint induced cell-cycle arrest and increase genomic instability.Synonyms: Cell cycle checkpoint control protein RAD9A, DNA repair exonuclease rad9 homolog A, EC=3.1.11.2, RAD-9A

NCBI Accession: NP_004575

UniProt: Q99638

Pathways: Positive Regulation of Response to DNA Damage Stimulus