BRCC3 antibody (N-Term)

Catalog No. ABIN318911

The Rabbit Polyclonal anti-BRCC3 antibody is suitable to detect BRCC3 in samples from Human, Mouse and Rat. It has been validated for WB and IHC (p).

Quick Overview for BRCC3 antibody (N-Term) (ABIN318911)

Target: BRCC3 (BRCA1/BRCA2-Containing Complex, Subunit 3 (BRCC3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRCC3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-BRCC3 Antibody

Binding Specificity: N-Term

Specificity: This antibody recognises human BRCA1/BRCA2 complex subunit 36 (BRCC36), a 36.7 kDa nuclear protein.

Purification: Purified

Immunogen: An 18 amino acid peptide from near the amino terminus of human BRCC36.

Isotype: IgG

Application Details

Application Notes: Western Blot: 0.5 - 1.0g/mL, detects a band of approximately 36 kDa in MCF7 cell lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline containing 0.02 % Sodium Azide (NaN3)

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for BRCC3

Target: BRCC3 (BRCA1/BRCA2-Containing Complex, Subunit 3 (BRCC3))

Alternative Name: BRCC3

Background: BRCC36, together with BRCA1, BRCA2, Rad51 and BRCC45, forms a holoenzyme complex that possesses an ubiquitin E3 ligase activity. Studies have demonstrated that BRCC36 functions in enhancing cellular survival following DNA damage and that it is essential for ionizing radiation induced BRCA1 phosphorylation and nuclear foci formation. Research has shown that BRCC36 is aberrantly expressed in the majority of breast tumours indicating a potential role in the pathogenesis of the disease. It has been hypothesized that BRCC36 could be a potentially important target in the treatment of radiation-resistant breast tumours.Synonyms: BRCA1-A complex subunit BRCC36, BRCA1/BRCA2-containing complex subunit 3, BRCA1/BRCA2-containing complex subunit 36, BRCC36, BRISC complex subunit BRCC36, C6.1A, CXorf53, Lys-63-specific deubiquitinase BRCC36

Gene ID: 79184

NCBI Accession: NP_001018065

UniProt: P46736

Pathways: Positive Regulation of Response to DNA Damage Stimulus