SNCA antibody (pSer129)

Catalog No. ABIN318946

This Rabbit Polyclonal antibody specifically detects SNCA in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Monkey and Dog.

Quick Overview for SNCA antibody (pSer129) (ABIN318946)

Target: SNCA (Synuclein, alpha (SNCA))

Reactivity: Human, Mouse, Rat, Cow, Monkey, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SNCA antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SNCA Antibody

Binding Specificity: pSer129

Specificity: This antibody specifically recognises rat alpha synuclein when phosphorylated at Ser129.

Purification: Purified

Immunogen: Synthetic phosphopeptide corresponding to amino acid sequence surrounding phosphorylated serine 129 of Alpha Synuclein.

Isotype: IgG

Application Details

Application Notes: Western Blot: 1: 1000, detects a band of approximately 15 kDa in rat cortex lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM Hepes pH 7.5, 150 mM NaCl containing 0.09 % Sodium Azide (NaN3), 50 % Glycerol 0.01 % Bovine Serum Albumin

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for SNCA

Target: SNCA (Synuclein, alpha (SNCA))

Alternative Name: alpha-Synuclein / SNCA

Background: Alpha synuclein is a 140 amino acid, 15 kDa protein seen predominantly in the neocortex, hippocampus, substantia nigra, thalamus and cerebellum. Its normal cellular function has not been definitively determined, but it is thought to be involved in the regulation of neuronal plasticity, dopamine neurotransmission and other membrane-associated processes at the presynaptic terminal. Alpha synuclein is normally a soluble, unfolded protein, but it can aggregate at high concentrations to form insoluble fibrils which, in association with other proteins such as ubiquitin, neurofilament protein and alpha B crystalline, form Lewy bodies. Lewy bodies are a hallmark pathological feature of Parkinsons disease and other neurodegenerative diseases. Alpha synuclein has several possible phosphorylation sites, Ser129 in particular can be phosphorylated by Gproteincoupled receptor kinases. Studies suggest that this phosphorylation promotes formation of alpha synuclein filaments, with hyperphosphorylation of Ser129 seen in Lewy bodies.Synonyms: NACP, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, PARK1

Gene ID: 6622

NCBI Accession: NP_000336

UniProt: P37840

Pathways: Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Platelet-derived growth Factor Receptor Signaling, Negative Regulation of Transporter Activity, Regulation of long-term Neuronal Synaptic Plasticity