RIPK2 antibody (N-Term)

Catalog No. ABIN318972

The Rabbit Polyclonal anti-RIPK2 antibody (ABIN318972) specifically detects RIPK2 in WB, IF and IHC (fro). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for RIPK2 antibody (N-Term) (ABIN318972)

Target: RIPK2 (Receptor-Interacting Serine-threonine Kinase 2 (RIPK2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RIPK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-RIPK2 Antibody

Binding Specificity: N-Term

Specificity: This antibody recognises Human RIP2 (Receptor-interacting kinase 2), a 61.2 kDa cytoplasmic protein belonging to the protein kinase superfamily.

Purification: Purified

Immunogen: A peptide corresponding to amino acids near the amino terminus of human RIP2.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry on frozen sections: 10g/mL. Western Blot: 0.5 - 1.0g/mL, detects a band of approximately 60 kDa in A431 cell lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline containing 0.02 % Sodium Azide (NaN3)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for RIPK2

Target: RIPK2 (Receptor-Interacting Serine-threonine Kinase 2 (RIPK2))

Alternative Name: RIPK2

Background: RIP2, known also as RICK and CARDIAK, is a novel kinase that regulates TNF and Fas induced-apoptosis and is involved in the generation of the pro-inflammatory cytokine IL-1beta. Over expression of RIP2 has been shown to induce apoptosis, and the activation of NF-Kappa-B and JNK. RIP2 is widely expressed in a range of human tissues.Synonyms: CARD-containing IL-1 beta ICE-kinase, CARDIAK, RICK, RIP-2, RIP-like-interacting CLARP kinase, RIP2, Receptor-interacting protein 2, Receptor-interacting serine/threonine-protein kinase 2

Gene ID: 8767

NCBI Accession: NP_003812

UniProt: O43353

Pathways: TCR Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Toll-Like Receptors Cascades