FLIP antibody (C-Term)

Catalog No. ABIN318973

This Rabbit Polyclonal antibody specifically detects FLIP in WB, IF and IHC (fro). It exhibits reactivity toward Human and Mouse.

Quick Overview for FLIP antibody (C-Term) (ABIN318973)

Target: FLIP (CFLAR) (CASP8 and FADD-Like Apoptosis Regulator (CFLAR))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FLIP antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-FLIP Antibody

Binding Specificity: AA 191-209, C-Term

Specificity: This antibody recognises the apoptosis related protein designated FLIP (FLICE inhibitory protein).

Purification: Purified

Immunogen: A peptide corresponding to aa191 - 209 at the C-terminus of human FLIP delta/FLIPs form.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry on frozen sections: 2g/mL. Western Blot: 1: 1000, detects bands of approximately 35 kDa and 25 kDa in Jurkat celllysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline containing 0.02 % Sodium Azide (NaN3)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for FLIP

Target: FLIP (CFLAR) (CASP8 and FADD-Like Apoptosis Regulator (CFLAR))

Alternative Name: CFLAR / Casper / I-FLICE

Background: FLIP interacts with the adaptor protein FADD, and with Caspase-8 (FLICE) and Caspase-10 (FLICE2) to inhibit apoptosis induced by death receptors including CD95, DR3, TRAIL-R and TNFR1. FLICE inhibitory proteins were initially identified in virus and human and were accordingly designated as v-FLIPs and c-FLIPs. Four spice variants of c-FLIP have been identified and are classified as FLIP alpha, beta, delta and gamma. AHP1591 recognizes human and murine delta and gamma variants but not the alpha or beta, variants. FLIP is widely expressed in human tissues and a diverse range of cell lines.Synonyms: CASH, CASP8 and FADD-like apoptosis regulator, CASP8AP1, CLARP, Caspase homolog, Caspase-eight-related protein, Caspase-like apoptosis regulatory protein, Cellular FLICE-like inhibitory protein, FADD-like antiapoptotic molecule 1, FLAME-1, Inhibitor of FLICE, MACH-related inducer of toxicity, MRIT, Usurpin, c-FLIP

Gene ID: 8837

NCBI Accession: NP_001120655

UniProt: O15519

Pathways: Apoptosis, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development