EIF4G1 antibody (pSer1232)

Catalog No. ABIN319277

The Rabbit Polyclonal anti-EIF4G1 antibody is suitable to detect EIF4G1 in samples from Human. It has been validated for WB and IHC (p).

Quick Overview for EIF4G1 antibody (pSer1232) (ABIN319277)

Target: EIF4G1 (Eukaryotic Translation Initiation Factor 4 Gamma, 1 (EIF4G1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF4G1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-EIF4G1 Antibody

Binding Specificity: pSer1232

Specificity: EIF4G (phospho-Ser1232) Antibody AP08034PU detects endogenous levels of eIF4G only when phosphorylated at Serine 1232.

Purification: Immunoaffinity Chromatography: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human eIF4G around the phosphorylation site of Serine 1232 (P-V-SP-P-L).

Application Details

Application Notes: Western Blot (1/500-1/1000). Immunohistochemistry (1/50-1/100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at-20 °C.

Target Details for EIF4G1

Target: EIF4G1 (Eukaryotic Translation Initiation Factor 4 Gamma, 1 (EIF4G1))

Alternative Name: EIF4G1 / EIF4F

Background: EIF4G1 (eukaryotic translation Initiation Factor 4 Gamma 1) is a component of the protein complex eIF-4 which is involved in the recognition of the mRNA cap ATP-dependent unwinding of the 5'-terminal secondary structure and recruitment of mRNA to the ribosome. eIF4G plays a critical role in protein expression and is at the center of a complex regulatory network. Together with the cap-binding protein eIF4E, it recruits the small ribosomal subunit to the 5'-end of mRNA and promotes the assembly of a functional translation initiation complex which scans along the mRNA to the translation start codon. Human eIF4G contains three consecutive HEAT domains, as well as long unstructured regions involved in multiple protein-protein interactions. The interactions of eIF4G1 with other factors are largely unknown.Synonyms: EIF4G, EIF4GI, Eukaryotic translation initiation factor 4 gamma 1, eIF-4-gamma 1, eIF-4G 1, eIF-4G1, p220

Gene ID: 1981

NCBI Accession: NP_886553

UniProt: Q04637