EIF4G1 antibody (pSer1232)

Catalog No. ABIN319277

Product Details anti-EIF4G1 Antibody

Target: EIF4G1 (Eukaryotic Translation Initiation Factor 4 Gamma, 1 (EIF4G1))

Binding Specificity: pSer1232

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF4G1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: EIF4G (phospho-Ser1232) Antibody AP08034PU detects endogenous levels of eIF4G only when phosphorylated at Serine 1232.

Purification: Immunoaffinity Chromatography: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human eIF4G around the phosphorylation site of Serine 1232 (P-V-SP-P-L).

Application Details

Application Notes: Western Blot (1/500-1/1000). Immunohistochemistry (1/50-1/100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at-20 °C.

Target Details for EIF4G1

Target: EIF4G1 (Eukaryotic Translation Initiation Factor 4 Gamma, 1 (EIF4G1))

Alternative Name: EIF4G1 / EIF4F (EIF4G1 Products)

Synonyms: EIF4GI antibody, CG10811 antibody, Dmel\\CG10811 antibody, EIF4G antibody, Eif4G antibody, deIF4G antibody, eIF-4G antibody, eIF4g antibody, p200 antibody, EIF4G1 antibody, wu:fb50a05 antibody, wu:fc60b06 antibody, eif4g antibody, CUCUMOVIRUS MULTIPLICATION 2 antibody, CUM2 antibody, EUKARYOTIC TRANSLATION INITIATION FACTOR 4G antibody, eukaryotic translation initiation factor 4G antibody, DDBDRAFT_0217646 antibody, DDBDRAFT_0217647 antibody, DDBDRAFT_0234257 antibody, DDB_0217646 antibody, DDB_0217647 antibody, DDB_0234257 antibody, NV18824 antibody, EIF-4G1 antibody, EIF4F antibody, P220 antibody, PARK18 antibody, p220 antibody, E030015G23Rik antibody, eIF4GI antibody, eukaryotic translation initiation factor 4 gamma 1 antibody, eukaryotic translation initiation factor 4G antibody, eukaryotic translation initiation factor 4 gamma, 1a antibody, eukaryotic translation initiation factor 4 gamma, 1 S homeolog antibody, eukaryotic initiation factor antibody, eukaryotic translation initiation factor 4 gamma antibody, eukaryotic translation initiation factor 4 gamma, 1 antibody, eukaryotic translation initiation factor 4, gamma 1 antibody, EIF4G1 antibody, eIF4G antibody, eif4g1a antibody, LOC704923 antibody, eif4g1.S antibody, EIF4G antibody, eIF4g antibody, Eif4g antibody, Eif4g1 antibody

Background: EIF4G1 (eukaryotic translation Initiation Factor 4 Gamma 1) is a component of the protein complex eIF-4 which is involved in the recognition of the mRNA cap ATP-dependent unwinding of the 5'-terminal secondary structure and recruitment of mRNA to the ribosome. eIF4G plays a critical role in protein expression and is at the center of a complex regulatory network. Together with the cap-binding protein eIF4E, it recruits the small ribosomal subunit to the 5'-end of mRNA and promotes the assembly of a functional translation initiation complex which scans along the mRNA to the translation start codon. Human eIF4G contains three consecutive HEAT domains, as well as long unstructured regions involved in multiple protein-protein interactions. The interactions of eIF4G1 with other factors are largely unknown.Synonyms: EIF4G, EIF4GI, Eukaryotic translation initiation factor 4 gamma 1, eIF-4-gamma 1, eIF-4G 1, eIF-4G1, p220

Gene ID: 1981

NCBI Accession: NP_886553

UniProt: Q04637