CD44 antibody

Catalog No. ABIN319439

The Mouse Monoclonal anti-CD44 antibody has been validated for FACS, WB, IHC (p), IP and IHC (fro). It is suitable to detect CD44 in samples from Human and Monkey.

Quick Overview for CD44 antibody (ABIN319439)

Target: CD44

Reactivity: Human, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD44 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 156-3C11

Product Details anti-CD44 Antibody

Specificity: This antibody recognises CD44, a 90 kDa type I transmembrane protein which is otherwise known as HCAM or CD44s. Various isoforms of CD44 exist. The 90 kDa standard form, recognised by this antibody, is expressed on leucocytes, erythrocytes, white matter of the brain and some epithelial cells of the breast and small intestine. Antibodies produced by clone 156-3C11 recognise epitope 3, which is a protease resistant epitope on the CD44 Molecule.

Purification: Purified IgG prepared by affinity chromatography on Protein G

Immunogen: Stimulated human leucocytes.

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry: use 10 μL of 1/50-1/100 diluted antibody to label 1x10^6 cells in 100 μL. Immunohistochemistry on Frozen and Paraffin Sections: (1/100). This product requiresantigen retrieval using heat treatment prior to staining of paraffin sections, Sodium citratebuffer pH 6.0 is recommended for this purpose. Positive Control: Human tonsil. Immunoprecipitation. Western Blot: Detects a band of approximately 80 kDa in HeLa cell lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline pH 7.4 containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD44

Target: CD44

Alternative Name: CD44

Background: CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential.Synonyms: CDw44, ECMR-III, Epican, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, LHR, MDU2, MDU3, MIC4, PGP-1, Phagocytic glycoprotein 1

Gene ID: 960

NCBI Accession: NP_000601

UniProt: P16070

Pathways: Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling