CD34 antibody

Catalog No. ABIN319717

This Mouse Monoclonal antibody specifically detects CD34 in FACS, WB, IP, IHC (p) and IHC (fro). It exhibits reactivity toward Human and Monkey.

Quick Overview for CD34 antibody (ABIN319717)

Target: CD34

Reactivity: Human, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD34 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: QBEND-10

Product Details anti-CD34 Antibody

Specificity: This antibody recognises the Class II epitope on CD34. Ultrastructure studies, using an indirect immunogold technique on ultra thin sections of human umbilical artery, have revealed that this antigen is associated to a degree with the basoluminal surfaces of endothelium, a slight reaction with an intracytoplasmic vesicular compartment is shown. Predominant staining is on the endothelial cell membrane. Some cross reactions with vascular associated adventitia and some basement membranes. Clone QBEND-10 immunoprecipitates a glycoprotein of Mr 110 kDa expressed on haemopoietic cells and on the established myeloid leukemic cell line KG1a.

Purification: Affinity Chromatography on Protein A

Immunogen: Human endothelial cell membrane vesicles. Spleen cells from immunised NZB mice were fused with cells of the mouse NSO myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: Western Blot. Immunoprecipitation. Flow Cytometry: Use 10 μL of 1/100-1/200 diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections (1/500-1/1000): This product does not require antigen retrieval using heat treatment or protein digestion. However staining of paraffin embedded formalin fixed tissues may be enhanced by enzyme pre-treatment using pronase or heat treatment using citrate buffer. Recommended positive control: Tonsil, Bone Marrow.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD34

Target: CD34

Alternative Name: CD34

Background: CD34 is a single chain transmembrane glycoprotein of 105 - 120kD. Expressed on haemopoietic cells, including bone marrow and blood. It is expressed on the majority of cells in acute lymphoid leukaemia (ALL) as well as cells in acute myelogenous leukaemia (AML). It is also found on capillary endothelial cells. There are three classes of CD34 epitopes defined by differential sensitivity to enzymatic cleavage with glycoprotease from Pasteurella haemolytica and with neuraminidase.Synonyms: Hematopoietic progenitor cell antigen CD34, Hematopoietic progenitor cell marker

Gene ID: 947

NCBI Accession: NP_001020280

UniProt: P28906