AOX1/AOX2 antibody (C-Term)

Catalog No. ABIN3197483

Cited in 7+ publications. The Rabbit Polyclonal anti-AOX1/AOX2 antibody (ABIN3197483) specifically detects AOX1/AOX2 in IL and WB. The antibody is reactive with Physcomytrium Patens, Zea mays, Triticum aestivum, Tomato, Symplocarpus renifolius, Soybean, Rosa hybrida, Robinia pseudoacacia, Rape, Potato, Poa annua, Picea abies, Arabidopsis thaliana, Pea, Oryza sativa, Nicotiana tabacum, Lupinus luteus, Kandelia candel, Eriophorum vaginatum, Copepod (Tigriopus), Cabbage, Betula nana, Beet and Barley samples.

Quick Overview for AOX1/AOX2 antibody (C-Term) (ABIN3197483)

Target: AOX1/AOX2 (Alternative Oxidase 1/2 (AOX1/AOX2))

Reactivity: Physcomytrium Patens, Zea mays, Triticum aestivum, Tomato, Symplocarpus renifolius, Soybean, Rosa hybrida, Robinia pseudoacacia, Rape, Potato, Poa annua, Picea abies, Arabidopsis thaliana, Pea, Oryza sativa, Nicotiana tabacum, Lupinus luteus, Kandelia candel, Eriophorum vaginatum, Copepod (Tigriopus), Cabbage, Betula nana, Beet, Barley

Host: Rabbit

Clonality: Polyclonal

Application: Immunolocalization (IL), Western Blotting (WB)

Product Details anti-AOX1/AOX2 Antibody

Binding Specificity: C-Term

Purpose: Anti-AOX1/2 | Plant alternative oxidase 1 and 2

Cross-Reactivity (Details): Arabidopsis thaliana, Betula nana, Beta vulgaris, Brassica napus, Brassica oleracea, Kandelia candel, Eriphorum vaginatum, Glycine max, Hordeum vulgare, Lupinus luteus, Nicotiana tabacum, Oryza sativa, Picea abies, Pisum sativum, Poa annua, Robinia pseudoacacia, Rosa hybrida, Solanum lycopersicum, Solanum tuberosum, Symplocarpus renifolius, Physcomitrium patens, Tigriopus californicus, Triticum aestivum, Zea mays
Not reactive in: Candidia albicans, Chlamydomonas reinhardtii (use an antibody to algal AOX1, AS06 152), Stomolophus sp2

Predicted Reactivity: Aegilops tauschii,Brachypodium distachyon,Capsella rubella,Citrus sinensis,Citrus clementina,Corylus heterophylla,Crocus sativus,Cucumis sativus,Daucus carota,Hypericum perforatum,Lotus japonicus,Malus x domestica,Medicago truncatula,Medicago sativa,Mangifera indica,Naegleria gruberi (amoeba),Nelumbo nucifera,Nicotiana benthamiana,Oryza brachyantha,Populus tremula,Picea sitchensis,Pinus pinaster,Pyrus communis,Saccharum officinarum,Sauromatum venosum,Sorghum bicolor,Selaginella moellendorffii,Tetrahymena thermophila,Vigna radiata,Vigna unguiculata,Vitis vinifera

Purification: Serum

Immunogen: KLH-conjugated synthetic peptide derived from fully conserved C-terminal consensus motif from plant AOX isoforms including Arabidopsis thaliana AOX1A. UniProt: Q39219

Application Details

Application Notes: 1 : 750 (IL), 1 : 1000 for 10-20 μg of mitochondrial protein/lane detection (WB)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution, add 50 μL of sterile water

Buffer: Serum

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

References for anti-AOX1/AOX2 antibody (ABIN3197483)

Barreto, Okura, Neshich, Maia, Arruda: "Overexpression of UCP1 in tobacco induces mitochondrial biogenesis and amplifies a broad stress response." in: BMC plant biology, Vol. 14, pp. 144, (2014) (PubMed).

Tsaniklidis, Delis, Nikoloudakis, Katinakis, Aivalakis: "Low temperature storage affects the ascorbic acid metabolism of cherry tomato fruits." in: Plant physiology and biochemistry : PPB / Société française de physiologie végétale, Vol. 84, pp. 149-57, (2014) (PubMed).

Zubo, Potapova, Yamburenko, Tarasenko, Konstantinov, Börner: "Inhibition of the electron transport strongly affects transcription and transcript levels in Arabidopsis mitochondria." in: Mitochondrion, Vol. 19 Pt B, pp. 222-30, (2014) (PubMed).

Wang, Vanlerberghe: "A lack of mitochondrial alternative oxidase compromises capacity to recover from severe drought stress." in: Physiologia plantarum, (2013) (PubMed).

Kornfeld, Heskel, Atkin, Gough, Griffin, Horton, Turnbull: "Respiratory flexibility and efficiency are affected by simulated global change in Arctic plants." in: The New phytologist, Vol. 197, Issue 4, pp. 1161-72, (2013) (PubMed).

Zhang, Peck: "Simplified enrichment of plasma membrane proteins for proteomic analyses in Arabidopsis thaliana." in: Proteomics, Vol. 11, Issue 9, pp. 1780-8, (2011) (PubMed).

Wang, Li, Liu, He, Bi: "Nitric oxide increases mitochondrial respiration in a cGMP-dependent manner in the callus from Arabidopsis thaliana." in: Nitric oxide : biology and chemistry / official journal of the Nitric Oxide Society, (2010) (PubMed).

Target Details for AOX1/AOX2

Target: AOX1/AOX2 (Alternative Oxidase 1/2 (AOX1/AOX2))

Alternative Name: Plant alternative oxidase 1 and 2 (AOX1/2)

Background:

Background: Alternative oxidases (AOX) are quinol oxidases located in the inner mitochondrial membrane of plants. They function as terminal oxidases in the alternate electron transport pathway, oxidizing ubiquinone to reduce oxygen to water.

Additional information: Mitochondrion inner membrane marker. Possibly in the inner surface of the inner mitochondrial membrane.Protocol for a plant mitochondria preparation can be found here. In protein samples which are older than few months AOX enzyme can undergo intensive dimerization. Such preparations should not be used to work with this antibody.

Molecular Weight: 36-40 | 36-40 for Arabidopsis thaliana

Gene ID: 821806

UniProt: Q39219, O22049, Q7XBG9, Q7XT33