PC antibody

Catalog No. ABIN3197484

The Rabbit Polyclonal anti- antibody has been validated for WB and EM. It is suitable to detect in samples from Nicotiana tabacum, Oryza sativa, Pisum sativum, Potato, Spinach, Wild Cabbage and Zea mays. There are 2+ publications available.

Quick Overview for PC antibody (ABIN3197484)

Target: PC

Reactivity: Nicotiana tabacum, Oryza sativa, Pisum sativum, Potato, Spinach, Wild Cabbage, Zea mays

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Electron Microscopy (EM)

Product Details

Characteristics: Expected / apparent Molecular Weight of the Antigene: 10 kDa

Purification: serum

Immunogen: purified native plastocyanin from Spinacia oleracea P00289

Application Details

Application Notes: Recommended Dilution: 1 : 2000 with alkaline phosphatase (WB), 1 : 100 (IG) MW 10 kDa.
Cellular [compartment marker] of chloroplast thylakoid lumen

Comment:

plastocyanin runs abberant due to negative charge at 12-19 kDa on SDS-PAGE depending upon the system used. in 15 % gel the protein will run closer to its true MW than in 12 % gel. In some cases PC can be very acidic and run at twice of its MW.PC1 runs closer to 14 kDa while PC2 runs closer to 19 kDa. For good resolution adding fresh DTT to the sample buffer is recommended. PC2 is generally more abundant and it increases with Cu feeding. PC1 is expressed first after etiolated seedlings are placed in the light.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 10 µL of sterile water.

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

References

Perea-García, Garcia-Molina, Andrés-Colás, Vera-Sirera, Pérez-Amador, Puig, Peñarrubia: "Arabidopsis copper transport protein COPT2 participates in the cross talk between iron deficiency responses and low-phosphate signaling." in: Plant physiology, Vol. 162, Issue 1, pp. 180-94, (2013) (PubMed).

Sikorskaite, Rajamäki, Baniulis, Stanys, Valkonen: "Protocol: Optimised methodology for isolation of nuclei from leaves of species in the Solanaceae and Rosaceae families." in: Plant methods, Vol. 9, Issue 1, pp. 31, (2013) (PubMed).

Target Details

Target: PC

Background: AGI Code: At1g20340
Plastocyanin (PC) is a small Cu protein and a mobile electron carrier in the lumen of the thylkoids. PC interacts with the B/F complex and Photosystem I. Alternative name: DNA-damage-repair/toleration protein DRT112.

Molecular Weight: 10 kDa

UniProt: P00289