PC (C-Term) antibody

Catalog No. ABIN2788135

Product Details

Target: PC

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Sequence: SLPPLDLQAL EKELVDRHGE EVTPEDVLSA AMYPDVFAHF KDFTATFGPL

Predicted Reactivity: Cow: 86%, Dog: 86%, Guinea Pig: 79%, Horse: 86%, Human: 100%, Mouse: 86%, Pig: 86%, Rat: 86%

Characteristics: This is a rabbit polyclonal antibody against PC. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human PC

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1178 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details

Target: PC

Background: This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene.
Alias Symbols: PCB
Protein Interaction Partner: HUWE1, SUMO2, UBC, SUMO1, MDM2, HECW2, ADRB2, IFIT1, PRKCDBP, PPT2, TUFM, RPS3A, NACA, MTHFD1, ILF3, ILF2, CDH2, UBE2N, NPM1, MDH2, GOT2, GOT1,
Protein Size: 1178

Molecular Weight: 127 kDa

Gene ID: 5091

NCBI Accession: NM_001040716, NP_001035806

UniProt: P11498