Isocitrate Dehydrogenase antibody

Catalog No. ABIN3197487

This Rabbit Polyclonal antibody specifically detects Isocitrate Dehydrogenase in WB. It exhibits reactivity toward Arabidopsis thaliana, Pisum sativum, Potato, Tomato and Zea mays and has been mentioned in 3+ publications.

Quick Overview for Isocitrate Dehydrogenase antibody (ABIN3197487)

Target: Isocitrate Dehydrogenase (IDH)

Reactivity: Arabidopsis thaliana, Pisum sativum, Potato, Tomato, Zea mays

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Isocitrate Dehydrogenase antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Isocitrate Dehydrogenase Antibody

Cross-Reactivity (Details): No cross-reactivity with: Chlamydomonas reinhardtii

Predicted Reactivity: dicots including Brassica napus, Vitis vinifera, monocots including Oryza sativa, Zea mays

Characteristics: Expected / apparent Molecular Weight of the Antigene: 39 / 45 kDa (Arabidopsis thaliana)

Purification: serum

Immunogen: KLH-conjugated peptide 1 and peptide 2 conserved in all higher plants mitochondrial, NAD dependent isocitrate dehydrogenase subunits including Arabidopsis thaliana IDH-I Q8LFC0, At4g35260 and IDH-II P93032, At4g17130

Application Details

Application Notes: Recommended Dilution 1 : 5 000 with standard ECL (WB). Additional Information: Peptide used to elicit this antibody is not conserved in NADPH dependentanzymes, partially conserved across eukaryotic Idh subunits. Some conservationacross bacterial which contain the NAD-dependent form of Idh (as opposed to theNADP-dependent form).

Comment:

cellular [compartment marker] of mitochondrial matrix

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS pH 7.4

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

References for anti-Isocitrate Dehydrogenase antibody (ABIN3197487)

Rurek, Woyda-Ploszczyca, Jarmuszkiewicz et al.: "Biogenesis of mitochondria in cauliflower (Brassica oleracea var. botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, ..." in: Biochimica et biophysica acta, Vol. 1847, Issue 4-5, pp. 399-417, (2015) (PubMed).

Lee, Lee, Yoo, Duncan, Oh, Lee, Lee, Whelan, Hwang: "Mitochondrial targeting of the Arabidopsis F1-ATPase γ-subunit via multiple compensatory and synergistic presequence motifs." in: The Plant cell, Vol. 24, Issue 12, pp. 5037-57, (2013) (PubMed).

Szal, Jastrz?bska, Kulka, Lea: "Influence of mitochondrial genome rearrangement on cucumber leaf carbon and nitrogen metabolism." in: Planta, Vol. 232, Issue 6, pp. 1371-82, (2010) (PubMed).

Target Details for Isocitrate Dehydrogenase

Target: Isocitrate Dehydrogenase (IDH)

Background: AGI Code: At4g35260
Plant NADH dependent isocitrate dehydrogenase enzyme is located in mitochondrial matrix. This enzyme is classified as an oxidoreductase and its function is to catalyze a reaction in the citric acid cycle, specifically the sequential dehydrogenation and decarboxylation of isocitrate to form a-ketoglutarate. It removes hydrogens from its substrate, isocitrate. In addition to this process, it functions as a decarboxylase, removing a CO2 from the six-carbon substrate to form a five-carbon product mentioned above as a-ketoglutarate. There are two forms of this enzyme NADP+ and NAD+ dependent.

Molecular Weight: expected: 39 kDa, apparent: 45 kDa (Arabidopsis thaliana)

UniProt: P93032