AGO4 antibody

Catalog No. ABIN3197495

The Rabbit Polyclonal anti-AGO4 antibody has been validated for WB, IP, ICC and Co-IP. It is suitable to detect AGO4 in samples from Arabidopsis thaliana and Hyacinthus orientalis (Common hyacinth).

Quick Overview for AGO4 antibody (ABIN3197495)

Target: AGO4 (EIF2C4) (Eukaryotic Translation Initiation Factor 2C, 4 (EIF2C4))

Reactivity: Arabidopsis thaliana, Hyacinthus orientalis (Common hyacinth)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AGO4 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Co-Immunoprecipitation ( Co-IP)

Product Details anti-AGO4 Antibody

Cross-Reactivity (Details): Not reactive in: Brassica oleracea, Hordeum vulgare, Solanum lycopersicum, Zea mays, Vigna angularis

Predicted Reactivity: Arabidopsis thaliana

Characteristics: Expected / apparent Molecular Weight of the Antigene: 103 kDa
Antibody concentration is 0.4 mg/mL in 50% glycerol, PBS, 0.005% thimerosal,0.05% BSA, threfore the antibody sample will not freeze in -20°C.binds enDog (Canine)enous siRNAs, preference for 24nt siRNAs with 5' A.

Purification: serum

Immunogen: KLH-conjugated synthetic peptide chosen from Arabidopsis thaliana AGO4 sequence UniProt: Q9ZVD5 , TAIR: At2g27040.

Application Details

Application Notes: 1: 4000 (WB), 5 μg of antibody per 1 gram of a fresh tissue (IP), 1: 100 (ICC)

Comment:

AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure. Use a 6 % gel for protein separation, which is run longer to avoid a cross-reactivity at ca. 40 kDa.Note that the AGO4 antibody reacts with the NEB prestained protein marker.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water.

Buffer: Antibody concentration is 0.4 mg/mL in 50 % glycerol, PBS, 0.005 % thimerosal,0.05 % BSA. Please, add 125 µl of deionized water and let it stand in 4 °C overnight to mix. Afterwards warm the antibody sample up to RT and allow theglycerol to dissolve well. Mak

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Storage: -20 °C

Storage Comment: Store at -20°C, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur adhering to the cap or sides of the tubes.

Target Details for AGO4

Target: AGO4 (EIF2C4) (Eukaryotic Translation Initiation Factor 2C, 4 (EIF2C4))

Alternative Name: AGO4

Background: AGI Code: At2g27040
AGO4 (Argonaute 4) belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).

Molecular Weight: 103 kDa

UniProt: Q9ZVD5

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Cellular Glucan Metabolic Process