DCL1 antibody

Catalog No. ABIN7676210

The Rabbit Polyclonal anti-DCL1 antibody has been validated for WB. It is suitable to detect DCL1 in samples from Arabidopsis thaliana. There is 1 publication available.

Quick Overview for DCL1 antibody (ABIN7676210)

Target: DCL1 (CD302) (CD302)

Reactivity: Arabidopsis thaliana

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DCL1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DCL1 Antibody

Cross-Reactivity: Arabidopsis thaliana

Cross-Reactivity (Details): Not reactive in: Zea mays

Predicted Reactivity: Arabidopsis thaliana

Characteristics: Expected / apparent Molecular Weight of the Antigene: 213 kDa
DCL1 (EC=3.1.26) is a ribonuclease (RNase) III involved in RNA-mediated post-transcriptional gene silencing (PTGS). Functions in the microRNAs (miRNAs) biogenesis pathway by cleaving primary miRNAs (pri-miRNAs) and precursor miRNAs (pre-miRNAs). Synonymes: Protein ABNORMAL SUSPENSOR 1, Protein CARPEL FACTORY, Protein SHORT, INTEGUMENTS 1, Protein SUSPENSOR 1.

Purification: serum

Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana DCL1 sequence, Q9SP32 , At1g01040

Application Details

Application Notes: 1 : 1000 with standard ECL (WB)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water.

Storage: -20 °C

Storage Comment: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

References for anti-DCL1 antibody (ABIN7676210)

Yoshikawa: "Pre-microRNA processing activity in nuclear extracts from Arabidopsis suspension cells." in: Journal of plant research, (2016) (PubMed).

Target Details for DCL1

Target: DCL1 (CD302) (CD302)

Alternative Name: DCL1

Background: AGI Code: At1g01040
DCL1 (EC=3.1.26) is a ribonuclease (RNase) III involved in RNA-mediated post-transcriptional gene silencing (PTGS). Functions in the microRNAs (miRNAs) biogenesis pathway by cleaving primary miRNAs (pri-miRNAs) and precursor miRNAs (pre-miRNAs). Synonymes: Protein ABNORMAL SUSPENSOR 1, Protein CARPEL FACTORY, Protein SHORT, INTEGUMENTS 1, Protein SUSPENSOR 1.

Molecular Weight: 213 kDa

UniProt: Q9SP32

Pathways: Regulation of Actin Filament Polymerization