DCL1 antibody

Catalog No. ABIN7676210

There is 1 publication for this product available. The Rabbit Polyclonal anti-DCL1 antibody is suitable to detect DCL1 in samples from Arabidopsis thaliana. It has been validated for WB.

Quick Overview for DCL1 antibody (ABIN7676210)

Target: DCL1 (CD302) (CD302)

Reactivity: Arabidopsis thaliana

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DCL1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DCL1 Antibody

Purpose: DCL1 | Dicer-like protein 1

Cross-Reactivity: Arabidopsis thaliana

Cross-Reactivity (Details): Arabidopsis thaliana
Not reactive in: Zea mays

Predicted Reactivity: Arabidopsis thaliana

Purification: Antigen affinity purified serum

Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana DCL1 protein sequence

Application Details

Application Notes: 1 : 1000 (WB)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution, add 50 μL of sterile water.

Buffer: Antigen affinity purified serum in PBS, pH 7.4

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted, make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

References for anti-DCL1 antibody (ABIN7676210)

Yoshikawa: "Pre-microRNA processing activity in nuclear extracts from Arabidopsis suspension cells." in: Journal of plant research, (2016) (PubMed).

Target Details for DCL1

Target: DCL1 (CD302) (CD302)

Alternative Name: DCL1

Background:

Background: DCL1 (EC=3.1.26) is a ribonuclease (RNase) III involved in RNA-mediated post-transcriptional gene silencing (PTGS). Functions in the microRNAs (miRNAs) biogenesis pathway by cleaving primary miRNAs (pri-miRNAs) and precursor miRNAs (pre-miRNAs). Alternative names: Protein ABNORMAL SUSPENSOR 1, Protein CARPEL FACTORY, Protein SHORT, INTEGUMENTS 1, Protein SUSPENSOR 1.

Additional information: Freezing and re-using of diluted anti-DCL1 antibodies is not recommended and will contribute to non-conclusive results.

Molecular Weight: 213 kDa

UniProt: Q9SP32

Pathways: Regulation of Actin Filament Polymerization