Endoglin antibody

Catalog No. ABIN319844

The Mouse Monoclonal anti-Endoglin antibody has been validated for WB, FACS, IP and IHC (fro). It is suitable to detect Endoglin in samples from Human and Horse.

Quick Overview for Endoglin antibody (ABIN319844)

Target: Endoglin (ENG)

Reactivity: Human, Horse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Endoglin antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: SN6

Product Details anti-Endoglin Antibody

Specificity: This antibody recognises the CD105 cell surface antigen.

Purification: Affinity Chromatography on Protein G

Immunogen: Partially purified cell membrane antigens from fresh leukemia cells. Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3/NS1/1-Ag4-1 myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: Use 10 μL of 1/50 diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry on Frozen Sections. Western Blot. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Endoglin

Target: Endoglin (ENG)

Alternative Name: CD105 / Endoglin

Background: CD105 is a glycoprotein homodimer of 95kD subunits. CD105 is also known as endoglin, and is expressed by endothelial cells, activated monocytes and some leukaemia cells.Synonyms: END, ENG, HHT1, ORW, ORW1

Gene ID: 9606

UniProt: P17813