EID1 antibody (AA 159-187)

Catalog No. ABIN3201016

The Mouse Monoclonal anti-EID1 antibody has been validated for ELISA, WB and IF. It is suitable to detect EID1 in samples from Human. There are 2+ publications available.

Quick Overview for EID1 antibody (AA 159-187) (ABIN3201016)

Target: EID1 (EP300 Interacting Inhibitor of Differentiation 1 (EID1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EID1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF)

Clone: 26

Product Details anti-EID1 Antibody

Binding Specificity: AA 159-187

Specificity: Reacts with human, mouse and rat EID1 proteins

Purification: This antibody was purified by our propriety chromatography under mild conditions as IgG fraction from serum-free growth medium of hybridoma clone #26.

Sterility: Sterile filtered

Immunogen: Synthetic peptide containing aa 159-187 of human DIE1 protein

Isotype: IgG2a kappa

Application Details

Application Notes: 1) Western blotting: ~1 g/mL
2) Immunofluorescence staining: 1~5 g/mL
3) ELISA
Other applications have not been tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS ( pH 7.4), 50 % glycerol. Azide- and carrier-free

Preservative: Azide free

Storage: -20 °C

Storage Comment: Upon arrival centrifuge briefly and store at -20 C.

References for anti-EID1 antibody (ABIN3201016)

Nguyen, Baglia, Huang, Baker, McCance: "Acetylation regulates the differentiation-specific functions of the retinoblastoma protein." in: The EMBO journal, Vol. 23, Issue 7, pp. 1609-18, (2004) (PubMed).

MacLellan, Xiao, Abdellatif, Schneider: "A novel Rb- and p300-binding protein inhibits transactivation by MyoD." in: Molecular and cellular biology, Vol. 20, Issue 23, pp. 8903-15, (2000) (PubMed).

Target Details for EID1

Target: EID1 (EP300 Interacting Inhibitor of Differentiation 1 (EID1))

Alternative Name: EID1

Background: EID1 (EP300 interacting inhibitor of differentiation 1) encodes a 21 kDa protein, which interacts with RB1 and EP300 and acts as a repressor of MYOD1 transcription. EID1 inhibits EP300 and CBP histone acetyltransferase activity. It may be involved in coupling cell cycle exit to the transcription of genes required for cellular differentiation.

UniProt: Q9Y6B2