EID1 antibody (AA 40-120)

Catalog No. ABIN7214702

The Rabbit Polyclonal anti-EID1 antibody (ABIN7214702) specifically detects EID1 in ELISA, WB, IHC and IF. The antibody is reactive with Human and Mouse samples.

Quick Overview for EID1 antibody (AA 40-120) (ABIN7214702)

Target: EID1 (EP300 Interacting Inhibitor of Differentiation 1 (EID1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EID1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-EID1 Antibody

Binding Specificity: AA 40-120

Purpose: Rabbit Anti-EID-1 Polyclonal Antibody

Specificity: EID-1 Polyclonal Antibody detects endogenous levels of EID-1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human EID-1 at AA range: 40-120

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:10000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for EID1

Target: EID1 (EP300 Interacting Inhibitor of Differentiation 1 (EID1))

Alternative Name: EID-1

Background: EID1, C15orf3, CRI1, RBP21, PNAS-22, PTD014, EP300-interacting inhibitor of differentiation 1, 21 kDa pRb-associated protein, CREBBP/EP300 inhibitory protein 1, E1A-like inhibitor of differentiation 1, EID-1E1A-like inhibitor of differentiation-1 (EID-1), an acetyltransferase enzyme, binds both the retinoblastoma protein (Rb), a regulator of cell cycle and tissue specific transcription, and the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. EID-1 inhibits cellular differentiation by blocking the histone acetyltransferase activity of p300. EID-1 also acetylates both histones and non-histone proteins such as NCOA3 co-activator. By acetylating histones, EID-1 gives a specific tag for transcriptional activation. In addition to binding Rb and p300, EID-1 also binds to phosphorylated CREB protein, mediating cAMP gene regulation. EID-1 augments the activity of phosphorylated CREB and activates transcription of cAMP responsive genes as a co-activator.

Molecular Weight: 21kD

Gene ID: 23741

UniProt: Q9Y6B2