GNRH1 antibody (AA 25-92)

Catalog No. ABIN3201516

The Mouse Monoclonal anti-GNRH1 antibody has been validated for IHC, WB, IP and ICC. It is suitable to detect GNRH1 in samples from Rat.

Quick Overview for GNRH1 antibody (AA 25-92) (ABIN3201516)

Target: GNRH1 (Gonadotropin-Releasing Hormone 1 (Luteinizing-Releasing Hormone) (GNRH1))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This GNRH1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: 5-1#

Product Details anti-GNRH1 Antibody

Binding Specificity: AA 25-92

Purpose: Monoclonal Antibody to Gonadotropin Releasing Hormone (GnRH)

Sequence: MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- HWSYGL RPGGKRNTEH LVDSFQEMGK EEDQMAEPQN FECTVHWPRS PLRDLRGALE RLIEEEAGQK KM

Specificity: The antibody is a mouse monoclonal antibody raised against GnRH. It has been selected for its ability to recognize GnRH in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Gonadotropin Releasing Hormone (GnRH) corresdonding to His25~Met92 (Accession # P07490)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for GNRH1

Target: GNRH1 (Gonadotropin-Releasing Hormone 1 (Luteinizing-Releasing Hormone) (GNRH1))

Alternative Name: GnRH

Background: GNRH1, GRH, LNRH, LHRH, LH-RH I, Luliberin, Luteinizing-Hormone Releasing Hormone, Progonadoliberin-1, Gonadoliberin I, Gonadorelin, GnRH-associated peptide 1

Pathways: Hormone Activity