ALOX5 antibody (AA 461-671)

Catalog No. ABIN3201592

The Mouse Monoclonal anti-ALOX5 antibody (Clone D10) (ABIN3201592) specifically detects ALOX5 in WB. The antibody is reactive with Human samples.

Quick Overview for ALOX5 antibody (AA 461-671) (ABIN3201592)

Target: ALOX5 (Arachidonate 5-Lipoxygenase (ALOX5))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ALOX5 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: D10

Product Details anti-ALOX5 Antibody

Binding Specificity: AA 461-671

Purpose: Monoclonal Antibody to 5-Lipoxygenase (5-LO)

Sequence: MGHHHHHHSG SEF-ESKEDIPYYF YRDDGLLVWE AIRTFTAEVV DIYYEGDQVV EEDPELQDFV NDVYVYGMRG RKSSGFPKSV KSREQLSEYL TVVIFTASAQ HAAVNFGQYD WCSWIPNAPP TMRAPPPTAK GVVTIEQIVD TLPDRGRSCW HLGAVWALSQ FQENELFLGM YPEEHFIEKP VKEAMARFRK NLEAIVSVIA ERNKKKQLPY YYLSPDRIPN S

Specificity: The antibody is a mouse monoclonal antibody raised against 5-LO. It has been selected for its ability to recognize 5-LO in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Pig, Rat

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant 5-Lipoxygenase (5-LO) corresdonding to Glu461~Ser671 with N-terminal His Tag

Isotype: IgG2b kappa

Application Details

Application Notes: Western blotting: 0.01-2 μg/mLOptimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for ALOX5

Target: ALOX5 (Arachidonate 5-Lipoxygenase (ALOX5))

Alternative Name: 5-LO

Background: LOX5, 5-LOX, ALOX-5, ALOX5, 5LPG, LOG5, Lipoxygenase 5