Kdm6b antibody (AA 1613-1662)

Catalog No. ABIN320516

The Rabbit Polyclonal anti-Kdm6b antibody has been validated for WB. It is suitable to detect Kdm6b in samples from Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Pig and Monkey.

Quick Overview for Kdm6b antibody (AA 1613-1662) (ABIN320516)

Target: Kdm6b (Lysine (K)-Specific Demethylase 6B (Kdm6b))

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Pig, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Kdm6b antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Kdm6b Antibody

Binding Specificity: AA 1613-1662

Specificity: Human KDM6B / JMJD3

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog (100%).

Purification: Protein A purified

Immunogen: Synthetic peptide located between aa1613-1662 of human KDM6B (O15054). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Galago, Marmoset, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig (100%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1.25 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. This antibody is thought to recognize the 644 amino acid splice variant of JMJD3.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Kdm6b

Target: Kdm6b (Lysine (K)-Specific Demethylase 6B (Kdm6b))

Alternative Name: KDM6B / JMJD3

Background: Name/Gene ID: KDM6B

Synonyms: KDM6B, JMJD3, Jumonji domain containing 3, Lysine-specific demethylase 6B, KIAA0346, Lysine demethylase 6B

Gene ID: 23135

UniProt: O15054

Pathways: Warburg Effect