PKLR antibody (AA 56-105)

Catalog No. ABIN320669

The Rabbit Polyclonal anti-PKLR antibody has been validated for WB, IHC and IHC (p). It is suitable to detect PKLR in samples from Human, Mouse, Rat, Dog, Cow, Guinea Pig, Rabbit, Bat, Monkey and Xenopus laevis.

Quick Overview for PKLR antibody (AA 56-105) (ABIN320669)

Target: PKLR (Pyruvate Kinase, Liver and RBC (PKLR))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Rabbit, Bat, Monkey, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKLR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PKLR Antibody

Binding Specificity: AA 56-105

Specificity: Human PKLR

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Xenopus (100%) Guinea pig, Zebrafish (85%).

Purification: Protein A purified

Immunogen: Synthetic peptide located between aa56-105 of human PKLR (P30613-2, NP_870986). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Opossum, Xenopus, Salmon, Stickleback, Beetle (100%), Horse, Pufferfish, Zebrafish, Drosophila (92%), Orangutan, Panda, Cat, Pig, Guinea pig, Turkey, Chicken, Platypus (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P, WB

Usage: ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 5.0 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PKLR

Target: PKLR (Pyruvate Kinase, Liver and RBC (PKLR))

Alternative Name: PKLR

Background: Name/Gene ID: PKLR

Synonyms: PKLR, PKL, Pyruvate kinase, liver and RBC, Pyruvate kinase type L, Red cell/liver pyruvate kinase, RPK, PK1, PKR, Pyruvate kinase 1, Pyruvate kinase isozymes R/L, R-type/L-type pyruvate kinase, PKRL, Pyruvate kinase isozyme R/L

Gene ID: 5313

NCBI Accession: NP_870986

UniProt: P30613

Pathways: Ribonucleoside Biosynthetic Process