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Occludin antibody (N-Term)

This anti-Occludin antibody is a Rabbit Polyclonal antibody detecting Occludin in WB. Suitable for Human, Mouse, Rat, Pig, Dog, Rabbit, Guinea Pig, Horse, Hamster and Monkey.
Catalog No. ABIN320748

Quick Overview for Occludin antibody (N-Term) (ABIN320748)

Target

See all Occludin (OCLN) Antibodies
Occludin (OCLN)

Reactivity

  • 126
  • 63
  • 43
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  • 1
  • 1
Human, Mouse, Rat, Pig, Dog, Rabbit, Guinea Pig, Horse, Hamster, Monkey

Host

  • 95
  • 39
  • 3
  • 1
Rabbit

Clonality

  • 89
  • 49
Polyclonal

Conjugate

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  • 2
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  • 2
  • 2
  • 2
  • 2
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  • 2
  • 1
This Occludin antibody is un-conjugated

Application

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Western Blotting (WB)
  • Binding Specificity

    • 23
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    N-Term

    Specificity

    Human OCLN / Occludin

    Predicted Reactivity

    Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Rabbit, Horse, Pig, Guinea pig (100%) Bovine (92%).

    Purification

    Protein A purified

    Immunogen

    Synthetic peptide from N-Terminus of human OCLN (Q16625, NP_002529). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Dog, Rabbit, Horse, Pig, Guinea pig (100%), Panda, Bovine, Bat, Opossum, Platypus (92%).

    Type of Immunogen: Synthetic peptide

    Isotype

    IgG
  • Application Notes

    Approved: WB

    Usage: ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 2.5 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

    Comment

    Target Species of Antibody: Human

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Distilled Water.

    Concentration

    Lot specific

    Buffer

    Lyophilized from PBS with 2 % sucrose

    Handling Advice

    Avoid repeat freeze-thaw cycles.

    Storage

    4 °C,-20 °C

    Storage Comment

    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • Target

    Occludin (OCLN)

    Alternative Name

    OCLN / Occludin

    Background

    Name/Gene ID: OCLN

    Synonyms: OCLN, Occludin, BLCPMG

    Gene ID

    100506658

    NCBI Accession

    NP_002529

    UniProt

    Q16625

    Pathways

    Cell-Cell Junction Organization, Hepatitis C
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