14-3-3 zeta antibody (C-Term)

Catalog No. ABIN321366

This anti-14-3-3 zeta antibody is a Rabbit Polyclonal antibody detecting 14-3-3 zeta in WB. Suitable for Human, Mouse, Rat, Cow, Sheep, Chicken, Pig, Dog, Horse, Rabbit, Guinea Pig, Monkey and Hamster.

Quick Overview for 14-3-3 zeta antibody (C-Term) (ABIN321366)

Target: 14-3-3 zeta (YWHAZ)

Reactivity: Human, Mouse, Rat, Cow, Sheep, Chicken, Pig, Dog, Horse, Rabbit, Guinea Pig, Monkey, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This 14-3-3 zeta antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-14-3-3 zeta Antibody

Binding Specificity: C-Term

Specificity: Human YWHAZ / Phospholipase A2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Dog, Pig, Guinea pig, Chicken (100%) Xenopus (92%).

Purification: Protein A purified

Immunogen: Synthetic peptide from C-Terminus of human YWHAZ (P63104, NP_003397). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Hamster, Elephant, Dog, Cat, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Chicken, Platypus, Lizard (100%), Bat, Xenopus (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 5.0 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for 14-3-3 zeta

Target: 14-3-3 zeta (YWHAZ)

Alternative Name: YWHAZ / 14-3-3 Zeta

Background: Name/Gene ID: YWHAZ
Family: Phospholipase

Synonyms: YWHAZ, 14-3-3 protein zeta/delta, 14-3-3-zeta, 14-3-3 delta, 14-3-3 protein zeta, 14-3-3 zeta, Phospholipase A2, YWHAD, KCIP-1

Gene ID: 7534

NCBI Accession: NP_003397

UniProt: P63104

Pathways: Apoptosis, Hormone Transport, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location