SMAD7 antibody (N-Term)

Catalog No. ABIN324250

This Rabbit Polyclonal antibody specifically detects SMAD7 in WB. It exhibits reactivity toward Human, Rat and Monkey.

Quick Overview for SMAD7 antibody (N-Term) (ABIN324250)

Target: SMAD7 (SMAD, Mothers Against DPP Homolog 7 (SMAD7))

Reactivity: Human, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD7 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SMAD7 Antibody

Binding Specificity: N-Term

Specificity: Human SMAD7

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Mouse, Dog, Bovine, Pig (92%) Rat (84%).

Purification: Protein A purified

Immunogen: Synthetic peptide from N-Terminus of human SMAD7 (O15105, NP_005895). Percent identity by BLAST analysis: Human, Monkey, Marmoset (100%), Mouse, Dog, Bovine, Bat, Rabbit, Pig (92%), Galago (85%), Rat (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1.25 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for SMAD7

Target: SMAD7 (SMAD, Mothers Against DPP Homolog 7 (SMAD7))

Alternative Name: SMAD7

Background: Name/Gene ID: SMAD7

Synonyms: SMAD7, CRCS3, HSMAD7, MAD homolog 7, SMAD 7, MADH7, MADH8, Mothers against DPP homolog 7, Mothers against DPP homolog 8, MAD homolog 8, SMAD family member 7

Gene ID: 4092

NCBI Accession: NP_005895

UniProt: O15105

Pathways: Interferon-gamma Pathway, Cell-Cell Junction Organization