UBE2N antibody (C-Term)

Catalog No. ABIN324309

This anti-UBE2N antibody is a Rabbit Polyclonal antibody detecting UBE2N in WB. Suitable for Human, Rat, Mouse, Zebrafish (Danio rerio), Cow, Sheep, Rabbit, Horse, Guinea Pig, Drosophila melanogaster, Chicken, Xenopus laevis, Monkey and Pig.

Quick Overview for UBE2N antibody (C-Term) (ABIN324309)

Target: UBE2N (Ubiquitin-Conjugating Enzyme E2N (UBE2N))

Reactivity: Human, Rat, Mouse, Zebrafish (Danio rerio), Cow, Sheep, Rabbit, Horse, Guinea Pig, Drosophila melanogaster, Chicken, Xenopus laevis, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2N antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-UBE2N Antibody

Binding Specificity: C-Term

Specificity: Human UBE2N

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Sheep, Bovine, Rabbit, Guinea pig, Chicken, Xenopus, Zebrafish (100%).

Purification: Protein A purified

Immunogen: Synthetic peptide from C-Terminus of human UBE2N (P61088, NP_003339). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Elephant, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Zebra finch, Chicken, Platypus, Xenopus, Trout, Catfish, Salmon, Pike, Zebrafish, Drosophila, Slime mold, Beetle, Arabidopsis (100%), Sea squirt (92%), Rice (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 2.5 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for UBE2N

Target: UBE2N (Ubiquitin-Conjugating Enzyme E2N (UBE2N))

Alternative Name: UBE2N / UBC13

Background: Name/Gene ID: UBE2N

Synonyms: UBE2N, BLU, UbcH13, UbcH-ben, Ubiquitin carrier protein N, Yeast UBC13 homolog, UBC13, Ubiquitin-protein ligase N

Gene ID: 7334

NCBI Accession: NP_003339

UniProt: P61088

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Positive Regulation of Response to DNA Damage Stimulus, Ubiquitin Proteasome Pathway