Notch1 antibody (AA 2428-2556)

Catalog No. ABIN324717

This anti-Notch1 antibody is a Sheep Polyclonal antibody detecting Notch1 in WB, ELISA, FACS and ICC. Suitable for Human.

Quick Overview for Notch1 antibody (AA 2428-2556) (ABIN324717)

Target: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This Notch1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-Notch1 Antibody

Binding Specificity: AA 2428-2556

Specificity: Recognizes human Notch-1 intracellular domain. Species sequence homology: canine 83 % and mouse 89 %. < 1 % cross-reactivity with rhNotch-2 IC, rhNotch-3 IC, and rhNotch-4 IC.

Purification: Affinity purified

Immunogen: E. coli-derived recombinant human rhNotch-1 (aa 2428 - 2556).

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), Flo (50 μg/mL), ICC (10 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Suitable for use in for use in Western blot, Immunocytochemistry, Flow cytometry. and Direct ELISA. Western blot: 0.1-0.2 μg/mL. The detection limit for rhNotch-1 is ~0.5 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: 10 μg/mL to detect human Notch-1 in SAOS human osteosarcoma cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at RT and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at RT. After blocking, cells were incubated with diluted primary antibody for. 3 hours at RT followed by NorthernLights 557 donkey anti-sheep lgG at RT for 1 hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Flow cytometry: 50 μg/mL and add 10il of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled antibodies may be visualized by adding 10il of a 25 μg/mL stock solution of a secondary developing reagent such as anti-sheep lgG conjugated to a fluorochrome. Direct ELISA: 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human Notch-1. The detection limit is ~0.2 ng/well.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute, 0.5 mL sterile PBS to a concentration 0.2 mg/mL.

Concentration: Lot specific

Buffer: Lyophilized from PBS, 5 % trehalose. Reconstitute, 0.5 mL sterile PBS to a concentration 0.2 mg/mL.

Target Details for Notch1

Target: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Alternative Name: NOTCH1

Background: Name/Gene ID: NOTCH1

Synonyms: NOTCH1, Notch 1, TAN1

Gene ID: 4851

UniProt: P46531

Pathways: Notch Signaling, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development