Notch1 antibody (AA 2428-2556)

Catalog No. ABIN324719

The Mouse Monoclonal anti-Notch1 antibody has been validated for WB, ELISA and ICC. It is suitable to detect Notch1 in samples from Human.

Quick Overview for Notch1 antibody (AA 2428-2556) (ABIN324719)

Target: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Notch1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Product Details anti-Notch1 Antibody

Binding Specificity: AA 2428-2556

Specificity: Recognizes human Notch-1 IC.

Purification: Protein A/G purified

Immunogen: E. coli-derived rhNotch-1 IC. (aa 2428 - 2556).

Type of Immunogen: Recombinant protein

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), ICC (10 μg/mL), WB (1 - 2 μg/mL)

Usage: Suitable for use in Direct ELISA, Western Blot and Immunocytochemistry. Direct ELISA: 0.5-1.0 μg/mL detection limit for rhNotch-1 IC is ~3 ng/well. Western Blot: 1-2 μg/mL detection limit for rhNotch-1 IC is ~25 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: 10 μg/mL. For SAOS2 osteosarcoma cells, cells were fixed with PBS containing 4 % paraformaldehyde for 20 min at RT and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100 and 1 % BSA for 45 min at RT. After blocking, cells were incubated with primary antibody overnight at 4?C followed by fluorochrome-coupled anti-mouse IgG at RT in the dark for 1 hour. Between each step, cells were washed with PBS containing 0.1 % BSA.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS

Concentration: Lot specific

Buffer: Lyophilized, PBS, 5 % trehalose

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Notch1

Target: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Alternative Name: NOTCH1

Background: Name/Gene ID: NOTCH1

Synonyms: NOTCH1, Notch 1, TAN1

Gene ID: 4851

UniProt: P46531

Pathways: Notch Signaling, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development