RPE65 antibody

Catalog No. ABIN325738

This Mouse Monoclonal antibody specifically detects RPE65 in WB, ELISA, IF, IHC, ICC and IHC (fro). It exhibits reactivity toward Human, Rat, Mouse, Cow and Pig.

Quick Overview for RPE65 antibody (ABIN325738)

Target: RPE65 (Retinal Pigment Epithelium-Specific Protein 65kDa (RPE65))

Reactivity: Human, Rat, Mouse, Cow, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This RPE65 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-RPE65 Antibody

Specificity: Recognizes RPE65. Species cross-reactivity: Bovine, porcine, human and mouse.

Purification: Protein A purified

Immunogen: Bovine RPE microsomal membrane proteins.

Type of Immunogen: Recombinant protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA, ICC, IF (1:250), IHC, IHC-Fr, WB (1:5000 - 1:10000)

Usage: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorescence, and Immunocytochemistry. Western Blot 1:5000-1:10000, recognizes a band at ~65kD representing bovine RPE65 protein. Also been tested on recombinant human protein in COS7 cells. Immunohistochemistry (Frozen): 1:250 on murine retinal sections and ICC in porcine. Immunofluorescence: 1:250.

Comment:

Target Species of Antibody: Bovine

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for RPE65

Target: RPE65 (Retinal Pigment Epithelium-Specific Protein 65kDa (RPE65))

Alternative Name: RPE65

Background: Name/Gene ID: RPE65

Synonyms: RPE65, LCA2, RBP-binding membrane protein, Retinol isomerase, SRPE65, Rd12, MRPE65, Retinoid isomerohydrolase, RP20

Gene ID: 6121

UniProt: Q16518