BrdU antibody (Biotin)

Catalog No. ABIN6757023

The Biotin-conjugated Sheep Polyclonal anti-BrdU antibody (ABIN6757023) specifically detects BrdU in IHC, IHC (p), IHC (fro), WB and ELISA.

Quick Overview for BrdU antibody (Biotin) (ABIN6757023)

Target: BrdU (Bromodeoxyuridine (BrdU))

Host: Sheep

Clonality: Polyclonal

Conjugate: This BrdU antibody is conjugated to Biotin

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB), ELISA

Product Details anti-BrdU Antibody (Biotin)

Specificity: Recognizes bromodeoxyuridine in all species since nucleotide is omni-present. Very low cross-reactivity to 5-methyl cytosine.

Purification: Protein G purified

Immunogen: Bromodeoxyuridine (KLH coupled).

Type of Immunogen: Hapten

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (1:200 - 1:20000), IHC, IHC-Fr, IHC-P, WB (1:50 - 1:500)

Usage: Suitable for use in Immunohistochemistry, ELISA and Western Blot. Immunohistochemistry: 10 μg/mL (frozen and paraffin sections). Denature DNA sample prior to use. Western Blot: 1:50-1:500. ELISA: 1:200-1:20000 against 1 μg/mL BrdU analyte. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 40 % glycerol. No BSA, no preservative added.

Preservative: Without preservative

Target Details for BrdU

Target: BrdU (Bromodeoxyuridine (BrdU))

Alternative Name: BrdU

Target Type: Chemical