GNLY antibody (Biotin)

Catalog No. ABIN330555

The Biotin-conjugated Goat Polyclonal anti-GNLY antibody (ABIN330555) specifically detects GNLY in WB and FACS. The antibody is reactive with Human samples.

Quick Overview for GNLY antibody (Biotin) (ABIN330555)

Target: GNLY (Granulysin (GNLY))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This GNLY antibody is conjugated to Biotin

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-GNLY Antibody (Biotin)

Specificity: Recognizes human Granulysin. Cross-reactivity: <1 % with rhNKG-2D.

Purification: Immunoaffinity purified

Immunogen: NS0-derived recombinant human rhGranulysin.

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: Flo (25 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Suitable for use in Western blot and Flow cytometry. Western blot: 0.1-0.2 μg/mL The detection limit is ~5 ng/lane under non-reducing and 20 ng/lane under reducing conditions. In this format, this. antibody shows less than 1 % cross-reactivity with rhNKG-2D. Flow cytometry: 25 μg/mL and add 10il of the diluted solution to 1-2.5 x 10^5 cells. Total reaction volume should not exceed 200 μL. Use PMA plus Ca 2+ ionomycin-activated PBMC. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute, 1 mL 0.1 % BSA in TBS to a concentration of 0.05 mg/mL.

Concentration: Lot specific

Buffer: Lyophilized from PBS, BSA. Reconstitute, 1 mL 0.1 % BSA in TBS to a concentration of 0.05 mg/mL.

Target Details for GNLY

Target: GNLY (Granulysin (GNLY))

Alternative Name: GNLY / Granulysin

Background: Name/Gene ID: GNLY

Synonyms: GNLY, 519, Granulysin, LAG2, LAG-2, Protein NKG5, Lymphocyte-activation gene 2, T-cell activation protein 519, TLA519, NKG5, D2S69E, Lymphokine LAG-2

Gene ID: 10578

UniProt: P22749