IDUA antibody (AA 26-653)

Catalog No. ABIN331022

The Sheep Polyclonal anti-IDUA antibody has been validated for ELISA, WB and IP. It is suitable to detect IDUA in samples from Human.

Quick Overview for IDUA antibody (AA 26-653) (ABIN331022)

Target: IDUA (Iduronidase, alpha-L- (IDUA))

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This IDUA antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-IDUA Antibody

Binding Specificity: AA 26-653

Specificity: Recognizes human IDUA.

Purification: Immunoaffinity purified

Immunogen: NS0-derived rhIDUA. (aa 26 - 653).

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), IP, WB (0.1 - 0.2 μg/mL)

Usage: Suitable for use in Direct ELISA, Western Blot and Immunoprecipitation. Direct ELISA: 0.5-1.0 μg/mL, Detection limit for rhIDUA is ~0.2 ng/well. Western Blot: 0.1-0.2 μg/mL, Detection limit for rhIDUA is ~5 ng/lane under non-reducing and reducing conditions. Antibody tested in WB and ELISA using the full-length recombinant protein. Immunoprecipitation: This antibody has been used to Immunoprecipitation rhIDUA from conditioned media of transfected NSO cells.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 500 μL sterile PBS

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.4, 5 % trehalose.

Storage: -20 °C

Storage Comment: Store at -20°C lyophilized. Once reconstituted, aliquot and store at -20°C.

Target Details for IDUA

Target: IDUA (Iduronidase, alpha-L- (IDUA))

Alternative Name: IDUA / MPS1

Background: Name/Gene ID: IDUA

Synonyms: IDUA, Alpha-L-iduronidase, MPS1, Iduronidase, alpha-L-, IDA

Gene ID: 3425

UniProt: P35475

Pathways: Glycosaminoglycan Metabolic Process