Actin antibody

Catalog No. ABIN331865

The Mouse Monoclonal anti-Actin antibody has been validated for WB, IHC, IHC (p) and IP. It is suitable to detect Actin in samples from Human, Rat, Mouse, Chicken and Rabbit.

Quick Overview for Actin antibody (ABIN331865)

Target: Actin

Reactivity: Human, Rat, Mouse, Chicken, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Product Details anti-Actin Antibody

Specificity: Reacts with all six known vertebrate isoforms of actin (MW ~42kD). It also reacts with two cytoplasmic actins (beta, gamma) which are highly homologous to one another, but which differ from the muscle actins by about 25 amino acid residues. This antibody is highly recommended for monitoring total protein load on Western blots. Species cross-reactivity: Human, bovine, mouse, canine, porcine, rabbit, rat, chicken, Dictyostelium discoideum, Physarum polycephalum.

Purification: Protein G purified

Immunogen: Chicken gizzard actin.

Type of Immunogen: Purified protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: IHC, IHC-P (0.5 μg/mL), IP, WB (0.5 μg/mL)

Usage: Suitable for use in Immunoprecipitation, Immunohistochemistry and Western Blot. Immunoprecipitation (Denatured verified): 2 μg/mg protein lysate. Use Protein G. Western Blot: 0.5 μg/mL for 2hrs at room temperature. Immunohistochemistry (formalin/paraffin): 1:400 for 30 min. Staining of formalin-fixed, paraffin-embedded tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min. followed by cooling at RT for 20 min. Positive control: HeLa cells, skeletal muscle. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Chicken

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM PBS,  pH 7.4.

Preservative: Azide free

Target Details for Actin

Target: Actin