PDPK1 antibody (AA 421-436)

Catalog No. ABIN334098

This anti-PDPK1 antibody is a Rabbit Polyclonal antibody detecting PDPK1 in WB, IHC and IHC (p). Suitable for Human, Mouse, Rat, Monkey, Pig and Dog.

Quick Overview for PDPK1 antibody (AA 421-436) (ABIN334098)

Target: PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Reactivity: Human, Mouse, Rat, Monkey, Pig, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDPK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PDPK1 Antibody

Binding Specificity: AA 421-436

Specificity: Recognizes human PDK-1 at ~48kD.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Elephant, Panda, Bovine, Dog, Bat, Horse, Pig (100%) Mouse, Rat, Hamster, Platypus (94%) Opossum, Chicken, Xenopus, Stickleback, Zebrafish (88%).

Purification: Protein A purified

Immunogen: Synthetic peptide corresponding to a sequence from human PDK-1 (KLH).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (1:1000)

Usage: Suitable for use in Western Blot and Immunohistochemistry. Western Blot (ECL): 1:1000. Positive control: Rat Brain Tissue Extract.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for PDPK1

Target: PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Alternative Name: PDK1

Background: Name/Gene ID: PDK1
Subfamily: PDK/BCKDK
Family: Protein Kinase

Synonyms: PDK1, PDHK, PDH kinase 1, PDHK1

Gene ID: 5163

UniProt: Q15118

Pathways: PI3K-Akt Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals