PDPK1 antibody (AA 421-436)

Catalog No. ABIN334098

The Rabbit Polyclonal anti-PDPK1 antibody (ABIN334098) specifically detects PDPK1 in WB, IHC and IHC (p). The antibody is reactive with Human, Mouse, Rat, Monkey, Pig and Dog samples.

Quick Overview for PDPK1 antibody (AA 421-436) (ABIN334098)

Target: PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Reactivity: Human, Mouse, Rat, Monkey, Pig, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDPK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PDPK1 Antibody

Binding Specificity: AA 421-436

Specificity: Recognizes human PDK-1 at ~48kD.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Elephant, Panda, Bovine, Dog, Bat, Horse, Pig (100%) Mouse, Rat, Hamster, Platypus (94%) Opossum, Chicken, Xenopus, Stickleback, Zebrafish (88%).

Purification: Protein A purified

Immunogen: Synthetic peptide corresponding to a sequence from human PDK-1 (KLH).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (1:1000)

Usage: Suitable for use in Western Blot and Immunohistochemistry. Western Blot (ECL): 1:1000. Positive control: Rat Brain Tissue Extract.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for PDPK1

Target: PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Alternative Name: PDK1

Background: Name/Gene ID: PDK1
Subfamily: PDK/BCKDK
Family: Protein Kinase

Synonyms: PDK1, PDHK, PDH kinase 1, PDHK1

Gene ID: 5163

UniProt: Q15118

Pathways: PI3K-Akt Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals