S100A8 antibody (FITC)

Catalog No. ABIN334255

This Mouse Monoclonal antibody specifically detects S100A8 in IHC, FACS and IHC (p). It exhibits reactivity toward Human, Rat, Cow, Pig, Rabbit, Dog, Cat, Monkey and Horse.

Quick Overview for S100A8 antibody (FITC) (ABIN334255)

Target: S100A8 (S100 Calcium Binding Protein A8 (S100A8))

Reactivity: Human, Rat, Cow, Pig, Rabbit, Dog, Cat, Monkey, Horse

Host: Mouse

Clonality: Monoclonal

Conjugate: This S100A8 antibody is conjugated to FITC

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-S100A8 Antibody (FITC)

Brand: IHC-plus™

Specificity: Recognizes human L1 Protein or Calprotectin Molecule. Variable results have been reported for the staining of brain macrophages and microglia. Species cross-reactivity: equine, Cynomolgus monkey, Rhesus monkey, monkey, bovine, baboon, rabbit, canine, feline, porcine, guinea pig and rat.

Purification: Protein G purified

Immunogen: Human monocytes.

Type of Immunogen: Cells

Isotype: IgG1

Application Details

Application Notes: Approved: Flo, IHC, IHC-P (1:50)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:50. Flow Cytometry: Neat. Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Membrane permeabilization is required. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 1 % BSA, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for S100A8

Target: S100A8 (S100 Calcium Binding Protein A8 (S100A8))

Alternative Name: S100A8 / MRP8

Background: Name/Gene ID: S100A8

Synonyms: S100A8, 60B8AG, CFAG, CAGA, Calgranulin A, Calgranulin-A, Calprotectin L1L subunit, Cystic fibrosis antigen, CGLA, L1Ag, NIF, p8, Protein S100-A8, MRP8, Urinary stone protein band A, CP-10, MA387, MRP-8

Gene ID: 6279

UniProt: P05109

Pathways: Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins