EEF2 antibody (pThr56)

Catalog No. ABIN334266

This anti-EEF2 antibody is a Rabbit Polyclonal antibody detecting EEF2 in WB, IHC, ELISA and IHC (p). Suitable for Human, Mouse, Rat, Chicken, Monkey and Hamster.

Quick Overview for EEF2 antibody (pThr56) (ABIN334266)

Target: EEF2 (Eukaryotic Translation Elongation Factor 2 (EEF2))

Reactivity: Human, Mouse, Rat, Chicken, Monkey, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EEF2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-EEF2 Antibody

Binding Specificity: pThr56

Specificity: Detects endogenous levels of eEF2 only when phosphorylated at threonine 56. It does not recognize eEF2 phosphorylated at other sites.

Purification: Immunoaffinity purified

Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Thr56 of human eEF2.

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (1:100), WB (1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:100. Western Blot: 1:1000, Incubate membrane with diluted antibody in 5 % BSA, 1X TBS, 0.1 % Tween-20 at 4uC with gentle shaking, overnight.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Preservative: Without preservative

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for EEF2

Target: EEF2 (Eukaryotic Translation Elongation Factor 2 (EEF2))

Alternative Name: EEF2 / Elongation Factor 2

Background: Name/Gene ID: EEF2

Synonyms: EEF2, EEF-2, EF-2, Elongation factor 2, Polypeptidyl-tRNA translocase, EF2

Gene ID: 1938

UniProt: P13639

Pathways: AMPK Signaling