SH3GLB1 antibody

Catalog No. ABIN334268

The Rabbit Polyclonal anti-SH3GLB1 antibody is suitable to detect SH3GLB1 in samples from Human, Mouse and Rat. It has been validated for WB, IHC and IHC (p).

Quick Overview for SH3GLB1 antibody (ABIN334268)

Target: SH3GLB1 (SH3-Domain GRB2-Like Endophilin B1 (SH3GLB1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SH3GLB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SH3GLB1 Antibody

Specificity: The antibody detects 40 kD BIF-1 in samples from human, mouse, and rat origins. Jurkat cell lysate and rat kidney tissue lysates can be used as positive controls.

Purification: Protein A purified

Immunogen: Synthetic peptide surrounding amino acid 351 of human BIF.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 - 5 μg/mL), WB (2 - 6 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5-5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 50 % glycerol, 1 % BSA, 0.02 % Thimerosal.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Short term: -20°C
Long term: -70°C.

Target Details for SH3GLB1

Target: SH3GLB1 (SH3-Domain GRB2-Like Endophilin B1 (SH3GLB1))

Alternative Name: SH3GLB1 / Bif / Endophilin B1

Background: Name/Gene ID: SH3GLB1
Family: Apoptosis

Synonyms: SH3GLB1, CGI-61, Endophilin B1, Endophilin-B1, PPP1R70, Bax-interacting factor 1, Bif-1, DJ612B15.2, KIAA0491, SH3-containing protein SH3GLB1

Gene ID: 51100

UniProt: Q9Y371

Pathways: Autophagy