TNNI3 antibody

Catalog No. ABIN334276

This Mouse Monoclonal antibody specifically detects TNNI3 in WB, IHC, ELISA and IHC (p). It exhibits reactivity toward Human, Mouse, Cow, Rabbit, Chicken and Frog.

Quick Overview for TNNI3 antibody (ABIN334276)

Target: TNNI3 (Cardiac Troponin I (TNNI3))

Reactivity: Human, Mouse, Cow, Rabbit, Chicken, Frog

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNNI3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-TNNI3 Antibody

Specificity: Recognizes bovine Troponin I

Purification: Protein A purified

Immunogen: Native bovine cardiac Troponin I.

Isotype: IgG2b

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (2.5 - 5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5-5 μg/mL.

Comment:

Target Species of Antibody: Bovine

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C (no longer than 1 week)
Long term -20°C
Aliquot to avoid freeze/thaw cycles.

Target Details for TNNI3

Target: TNNI3 (Cardiac Troponin I (TNNI3))

Alternative Name: TNNI3 / CTnI

Background: Name/Gene ID: TNNI3

Synonyms: TNNI3, CTnI, CMD1FF, CMD2A, RCM1, Troponin I, cardiac, Troponin I, cardiac muscle, Troponin I type 3 (cardiac), Cardiac troponin I, CMH7

Gene ID: 7137

UniProt: P19429