ALOX15 antibody (Internal Region)

Catalog No. ABIN334342

This Goat Polyclonal antibody specifically detects ALOX15 in WB, IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for ALOX15 antibody (Internal Region) (ABIN334342)

Target: ALOX15 (Arachidonate 15-Lipoxygenase (ALOX15))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ALOX15 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-ALOX15 Antibody

Binding Specificity: Internal Region

Purpose: ALOX15

Sequence: HYKTDVAVKD DPE

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-HYKTDVAVKDDPE, from the internal region of the protein sequence according to NP_001131.3.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Skeletal Muscle and Kidney. Recommended concentration: 2 μg/mL.

Western Blot: Approx. 80 kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 74.8 kDa according to NP_001131.3). Recommended concentration: 0.2-0.5 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ALOX15

Target: ALOX15 (Arachidonate 15-Lipoxygenase (ALOX15))

Alternative Name: ALOX15

Background: ALOX15, arachidonate 15-lipoxygenase, 15-LOX-1, 15-LOX2

Gene ID: 246

NCBI Accession: NP_001131

Pathways: Regulation of Actin Filament Polymerization