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alpha 2 Antiplasmin antibody (Internal Region)

Verified SERPINF2 Reactivity: Human WB, IHC, ELISA, IF, FACS Host: Goat Polyclonal unconjugated
Catalog No. ABIN334343
  • Target See all alpha 2 Antiplasmin (SERPINF2) Antibodies
    alpha 2 Antiplasmin (SERPINF2) (Alpha2 Antiplasmin (SERPINF2))
    Binding Specificity
    • 15
    • 8
    • 5
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivity
    • 55
    • 28
    • 18
    • 1
    • 1
    • 1
    Human
    Host
    • 52
    • 9
    • 3
    • 2
    • 1
    • 1
    Goat
    Clonality
    • 60
    • 8
    Polyclonal
    Conjugate
    • 36
    • 9
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This alpha 2 Antiplasmin antibody is un-conjugated
    Application
    • 47
    • 24
    • 16
    • 14
    • 13
    • 13
    • 11
    • 8
    • 8
    • 7
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
    Purpose
    Alpha2-antiplasmin
    Sequence
    KDFLQSLKGF PRGDK
    Specificity
    This antibody is expected to recognise both reported isoforms (NP_000925.2, NP_001159393.1). Reported variants represent identical protein (NP_000925.2, NP_001159392.1).
    Cross-Reactivity
    Human
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence C-KDFLQSLKGFPRGDK, from the internal region of the protein sequence according to NP_000925.2, NP_001159393.1.
    Isotype
    IgG
  • Application Notes

    Immunohistochemistry: In paraffin embedded Human Liver shows spotty cytoplasm staining in hepatocytes. Recommended concentration: 3-5 μg/mL.

    Western Blot: Approx 70 kDa band observed in lysates of cell line HepG2 (calculated MW of 54.6 kDa according to NP_000925.2). The observed molecular weight is observed by other sources. Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at r

    Peptide ELISA: antibody detection limit dilution 1:16000.

    Comment

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.<

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Abdul, Dekkers, Ariëns, Leebeek, Rijken, Uitte de Willige: "On the localization of the cleavage site in human alpha-2-antiplasmin, involved in the generation of the non-plasminogen binding form." in: Journal of thrombosis and haemostasis : JTH, Vol. 18, Issue 5, pp. 1162-1170, (2020) (PubMed).

  • Target
    alpha 2 Antiplasmin (SERPINF2) (Alpha2 Antiplasmin (SERPINF2))
    Alternative Name
    SERPINF2 (SERPINF2 Products)
    Synonyms
    A2AP antibody, AAP antibody, ALPHA-2-PI antibody, API antibody, PLI antibody, serpin antibody, AI747498 antibody, Pli antibody, Serpimf2 antibody, serpinf2 antibody, serpinf2l antibody, wu:fd57d08 antibody, Dmrs91 antibody, Pedf antibody, serpin family F member 2 antibody, serpin family F member 1 antibody, serine (or cysteine) peptidase inhibitor, clade F, member 2 antibody, serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2a antibody, SERPINF2 antibody, Serpinf2 antibody, SERPINF1 antibody, serpinf2a antibody, Serpinf1 antibody
    Background
    SERPINF2, serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2, A2AP, AAP, ALPHA-2-PI, API, PLI, alpha-2-plasmin inhibitor, serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment
    Gene ID
    5345
    NCBI Accession
    NP_000925, NP_001159393
    Pathways
    Regulation of Systemic Arterial Blood Pressure by Hormones
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