BHMT antibody (C-Term)

Catalog No. ABIN334358

The Goat Polyclonal anti-BHMT antibody has been validated for WB, IHC and ELISA. It is suitable to detect BHMT in samples from Human, Mouse and Rat. There are 4+ publications available.

Quick Overview for BHMT antibody (C-Term) (ABIN334358)

Target: BHMT (Betaine--Homocysteine S-Methyltransferase (BHMT))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This BHMT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-BHMT Antibody

Binding Specificity: C-Term

Purpose: BHMT

Sequence: EQQLKELFEK QK

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-EQQLKELFEKQK

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Liver and Kidney. Recommended concentration: 5 μg/mL.

Western Blot: Approx 45 kDa band observed in Rat Liver lysates (calculated MW of 44.9 kDa according to human NP_001704.1, 45.0 kDa according to mouse NP_057877.1 and rat NP_110477.1). This product has been successfully used in WB on Human (PMID: 27320863).

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-BHMT antibody (ABIN334358)

Kim, Kwon, Kim: "Alterations in the metabolomics of sulfur-containing substances in rat kidney by betaine." in: Amino acids, Vol. 46, Issue 4, pp. 963-8, (2014) (PubMed).

Yun, Ryu, Lee, Noh, Lee, Lee, Kang, Park, Kim, Kim: "Hepatic metabolism of sulfur amino acids in db/db mice." in: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, Vol. 53, pp. 180-6, (2013) (PubMed).

Yun, Ryu, Oh, Kim, Lee, Lee, Lee, Kim, Kim: "Plasma homocysteine level and hepatic sulfur amino acid metabolism in mice fed a high-fat diet." in: European journal of nutrition, Vol. 52, Issue 1, pp. 127-34, (2013) (PubMed).

Ryu, Kwak, Lee, Kang, Oh, Lee, Kim, Ma, Kim: "Sulfur amino acid metabolism in doxorubicin-resistant breast cancer cells." in: Toxicology and applied pharmacology, Vol. 255, Issue 1, pp. 94-102, (2011) (PubMed).

Target Details for BHMT

Target: BHMT (Betaine--Homocysteine S-Methyltransferase (BHMT))

Alternative Name: BHMT

Background: BHMT, betaine-homocysteine methyltransferase

Gene ID: 635, 12116, 81508

NCBI Accession: NP_001704

Pathways: Methionine Biosynthetic Process