BTLA antibody (C-Term)

Catalog No. ABIN334365

This anti-BTLA antibody is a Goat Polyclonal antibody detecting BTLA in WB and ELISA. Suitable for Mouse.

Quick Overview for BTLA antibody (C-Term) (ABIN334365)

Target: BTLA (B and T Lymphocyte Associated (BTLA))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This BTLA antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-BTLA Antibody

Binding Specificity: C-Term

Purpose: BTLA

Sequence: KEAPTEYASI CVRS

Specificity: This antibody is expected to recognize both reported isoforms (NP_861445.3, NP_001078826.1).

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence KEAPTEYASICVRS, from the C Terminus of the protein sequence according to NP_861445.3, NP_001078826.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx. 35 kDa band observed in Mouse Spleen lysates (calculated MW of 32.8 kDa according to Human NP_861445.3 and of 34.2 kDa according to Mouse NP_001032808.2). Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:4000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for BTLA

Target: BTLA (B and T Lymphocyte Associated (BTLA))

Alternative Name: BTLA

Background: BTLA, B and T lymphocyte associated, BTLA1, CD272, FLJ16065, MGC129743, B and T lymphocyte attenuator

Gene ID: 151888, 208154, 407756

NCBI Accession: NP_861445, NP_001078826

Pathways: Cancer Immune Checkpoints