CES1 antibody (Internal Region)

Catalog No. ABIN334372

This Goat Polyclonal antibody specifically detects CES1 in WB, IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for CES1 antibody (Internal Region) (ABIN334372)

Target: CES1 (Carboxylesterase 1 (CES1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CES1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-CES1 Antibody

Binding Specificity: Internal Region

Purpose: CES1

Sequence: NTQAAQKLKD KE

Specificity: This antibody is expected to recognise all three reported isoforms (NP_001020366.1, NP_001020365.1, NP_001257.4).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-NTQAAQKLKDKE, from the internal region (near C terminus) of the protein sequence according to NP_001020366.1, NP_001020365.1, NP_001257.4.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Lung shows staining of lymphocytes in the vasculature.. Recommended concentration, 2-3 μg/mL.

Western Blot: Approx 55-60 kDa band observed in Human Liver and Ileum lysates (calculated MW of 62.6 kDa according to NP_001020366.1). In transfected HEK293 transiently expressing CES1 a band of approx. 65 kDa is observed. This band is not observed in the n

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CES1

Target: CES1 (Carboxylesterase 1 (CES1))

Alternative Name: CES1

Background: CES1, carboxylesterase 1 (monocyte/macrophage serine esterase 1), ACAT, CEH, CES2, HMSE, HMSE1, MGC117365, PCE-1, SES1, TGH, acyl coenzyme A:cholesterol acyltransferase, carboxylesterase 1, carboxylesterase 2 (liver), cholesteryl ester hydrolase, egasyn,

Gene ID: 1066

NCBI Accession: NP_001020366, NP_001020365, NP_001257

Pathways: Monocarboxylic Acid Catabolic Process