CHRNA4 antibody (AA 29-43)

Catalog No. ABIN334375

This Goat Polyclonal antibody specifically detects CHRNA4 in WB and ELISA. It exhibits reactivity toward Human and Rat.

Quick Overview for CHRNA4 antibody (AA 29-43) (ABIN334375)

Target: CHRNA4 (Cholinergic Receptor, Nicotinic, alpha 4 (CHRNA4))

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This CHRNA4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-CHRNA4 Antibody

Binding Specificity: AA 29-43

Purpose: CHRNA4 (aa29-43)

Sequence: HVETRAHAEE RLLKK

Specificity: This antibody is expected NOT to cross-react with the similar alpha 2 subunit.

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-HVETRAHAEERLLKK, from the internal region of the protein sequence according to NP_000735.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 70 kDa band observed in Rat Brain lysates (calculated MW of 70.0 kDa according to Human NP_000735.1and of 70.2 kDa according to Rat NP_077330.1). Recommended concentration: 1-3 μg/mL. An additional band of unknown identity was also consi

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

This antibody was shown unfit for immune precipitation.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CHRNA4

Target: CHRNA4 (Cholinergic Receptor, Nicotinic, alpha 4 (CHRNA4))

Alternative Name: CHRNA4

Background: CHRNA4, cholinergic receptor, nicotinic, alpha 4, BFNC, EBN, EBN1, NACRA4,cholinergic receptor, nicotinic, alpha 4 subunit, cholinergic receptor, nicotinic, alpha polypeptide 4, neuronal acetylcholine receptor alpha 4 subunit, neuronal nicotinic acetylcho

Gene ID: 1137, 11438, 25590

NCBI Accession: NP_000735

Pathways: Synaptic Membrane