DDX19B antibody (Internal Region)

Catalog No. ABIN334385

The Goat Polyclonal anti-DDX19B antibody has been validated for WB and ELISA. It is suitable to detect DDX19B in samples from Human.

Quick Overview for DDX19B antibody (Internal Region) (ABIN334385)

Target: DDX19B (DEAD (Asp-Glu-Ala-As) Box Polypeptide 19B (DDX19B))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This DDX19B antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-DDX19B Antibody

Binding Specificity: Internal Region

Purpose: DBP5 / DDX19

Sequence: DLPVDKDGNP DNETY

Specificity: This antibody is expected to recognise DDX19A (NP_060802.1) and all reported isoforms of DDX19B (NP_009173.1, NP_001014451.1 and NP_001014449.1).

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DLPVDKDGNPDNETY, from the internal region of the protein sequence according to NP_009173.1, NP_001014451.1, NP_001014449.1, NP_060802.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 50 kDa band observed in lysates of human ovary and of cell line K562 (calculated MW of 50.5 kDa according to NP_001014451.1). Recommended concentration: 0.5-1.5 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:4000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for DDX19B

Target: DDX19B (DEAD (Asp-Glu-Ala-As) Box Polypeptide 19B (DDX19B))

Alternative Name: DDX19B

Background: DDX19, DBP5, DDX19B, DEAD (Asp-Glu-Ala-As) box polypeptide 19B, RNAh , ATP-dependent RNA helicase DDX19, DEAD (Asp-Glu-Ala-As) box polypeptide 19, DEAD-box RNA helicase DEAD5, DEAD-box protein 5, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 19, DEAD/H (As

Gene ID: 11269, 55308, 234733

NCBI Accession: NP_009173, NP_001014451, NP_001014449, NP_060802